NAD+ reconstitution calculator
Enter your NAD+ vial size and the bacteriostatic water you plan to add — the tool returns the resulting concentration and the exact units to draw on a U-100 syringe. Also known as NAD, Nicotinamide Adenine Dinucleotide.
NAD+ reconstitution reference
How much bacteriostatic water to add, and the concentration you end up with. Values are arithmetic — not a dosage recommendation.
Commonly reconstituted around 166.7 mg/mL in the published research literature.
| Vial | 1 mL water | 2 mL water | 3 mL water | 5 mL water |
|---|---|---|---|---|
| 1000 mg | 1000 mg/mL100 mg per 10 units | 500 mg/mL50 mg per 10 units | 333.333 mg/mL33.33 mg per 10 units | 200 mg/mL20 mg per 10 units |
On a U-100 insulin syringe, 1 mL = 100 units, so 10 units = 0.1 mL.
on a 30-unit (0.3 mL) U-100 insulin syringe (1 unit = 0.01 mL).
- Injection volume
- 0.05 mL
- Concentration
- 5 mg/mL
- Doses per vial
- 40 doses
This calculator is an educational reconstitution reference for research materials. It is not medical advice, a dosage recommendation, or a treatment claim. Always follow the guidance of a qualified professional.
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General handling reference for research materials — not medical or dosage advice.
- ReconstituteDraw the bacteriostatic water with a U-100 syringe and add it slowly down the vial wall — never squirt it straight onto the powder.
- DissolveSwirl gently to dissolve; never shake hard. Let it stand until the liquid runs clear.
- DrawDraw up to the number of units calculated above on a U-100 insulin syringe.
- StoreKeep lyophilized powder cool, dark or frozen; once reconstituted, refrigerate at 2–8 °C and use within about 2–4 weeks — follow the product label.
Storage after reconstitution
Keep the lyophilized NAD+ vial cool, dark, and sealed until you reconstitute it. Add the bacteriostatic water slowly down the inside wall of the vial rather than directly onto the powder, and swirl — do not shake. Once reconstituted, store at 2–8 °C and follow the product label for the usable window; most reconstituted research peptides are handled within about 2–4 weeks.
This page is a reconstitution and concentration reference for research material handling. It is not medical advice, not a dosage recommendation, and not a treatment claim.